Waters ACQUITY UPLC 色譜柱優點
- 超快速分離
- 超高分離度
- 超高靈敏度
- 行業**的設計與制造技術
- 容易從HPLC條件轉移到UPLC條件
- 使用壽命長，重現性好
第1個ACQUITY UPLC 顆粒是 1.7 μm 亞乙基橋雜化顆粒(BEH)，這是沃特世**代雜化顆粒，它是 UPLC 技術的一個關鍵推動力，共有 5 種不同的鍵合相：C18, C8, Shield RP18,Phenyl 和 HILIC。由于它是雜化顆粒，因此具有更寬的 pH 使用范圍(pH 1-12)，能夠幫助分離科學家快速有效地開發方法。同時，BEH 顆粒技術也用于 XBridge 系列的 HPLC色譜柱，有不同粒徑(2.5、3.5、5 和 10 μm)供選，因此分離方法能夠在 HPLC 和 UPLC 技術平臺實現無縫轉換。
第2個ACQUITY UPLC 顆粒是 1.8μm 的高強度硅膠顆粒(HSS)，共有三種不同的鍵合相：HSS C18 、HSS C18 SB (Selectivity for Bases)和 T3。HSS C18 是全封端的超高效通用型的C18 柱：為堿性化合物提供良好的峰形；同 ACQUITY UPLC BEH C18 柱相比有更強的保留能力；在酸性流動相條件下具有超長的使用壽命。HSS C18 SB(Selectivity for Bases)是未經封端處理的 C18 柱，設計用于低 pH 條件下方法開發，它提供互補的分離選擇性，特別針對堿性化合物，具有與現代高覆蓋率 C18 柱不同的分離選擇性。HSS T3 色譜柱是與 100 ％水性流動相兼容的 C18 柱，專門用來保留和分離極性水溶性好的有機小分子，很像 Atlantis T3 HPLC 柱。
Waters ACQUITY UPLC 色譜柱中UPLC顆粒技術參數
|
|
亞乙基橋雜化顆粒(BEH) |
高強度硅膠顆粒(HSS) |
|
粒徑 |
1.7 μm |
1.8 μm |
|
孔徑/孔體積 |
130? / 0.7 mL/g |
100? / 0.7 mL/g |
|
比表面積 |
185 m2/g |
230 m2/g |
|
*高耐壓 |
15000 psi (~1000 bar) |
15000 psi (~1000 bar) |
Waters ACQUITY UPLC 色譜柱中UPLC鍵合相技術參數
|
|
BEH顆粒 |
HSS顆粒 |
||||||
|
鍵合相 |
C18 |
C8 |
Shield RP 18 |
苯基 |
HILIC |
C18 |
C18 SB |
T3 |
|
碳載量 |
18% |
13% |
17% |
15% |
-- |
15% |
8% |
11% |
|
封尾 |
專有 |
專有 |
TMS |
專有 |
-- |
專有 |
無 |
專有 |
|
pH范圍 |
1-12 |
1-12 |
2-11 |
1-12 |
1-8 |
1-8 |
2-8 |
2-8 |
|
鍵合相
|
規格
|
粒徑
|
貨號
1根/包 |
貨號
3根/包 |
|
BEH C18
|
1.0×50mm
|
1.7μm
|
186002344
|
176000861
|
|
1.0×100mm
|
1.7μm
|
186002346
|
176000862
|
|
|
1.0×150mm
|
1.7μm
|
186002347
|
176001044
|
|
|
2.1×30mm
|
1.7μm
|
186002349
|
176001304
|
|
|
2.1×50mm
|
1.7μm
|
186002350
|
176000863
|
|
|
2.1×100mm
|
1.7μm
|
186002352
|
176000864
|
|
|
2.1×150mm
|
1.7μm
|
186002353
|
176001048
|
|
|
BEH Shield RP18
|
1.0×50mm
|
1.7μm
|
186002851
|
176000874
|
|
1.0×100mm
|
1.7μm
|
186002852
|
176000875
|
|
|
1.0×150mm
|
1.7μm
|
186003373
|
176001045
|
|
|
2.1×30mm
|
1.7μm
|
186003909
|
176001309
|
|
|
2.1×50mm
|
1.7μm
|
186002853
|
176000876
|
|
|
2.1×100mm
|
1.7μm
|
186002854
|
176000877
|
|
|
2.1×150mm
|
1.7μm
|
186003376
|
176001049
|
|
|
BEH C8
|
1.0×50mm
|
1.7μm
|
186002875
|
176000882
|
|
1.0×100mm
|
1.7μm
|
186002876
|
176000883
|
|
|
1.0×150mm
|
1.7μm
|
186003374
|
176001046
|
|
|
2.1×30mm
|
1.7μm
|
186003910
|
176001310
|
|
|
2.1×50mm
|
1.7μm
|
186002877
|
176000884
|
|
|
2.1×100mm
|
1.7μm
|
186002878
|
176000885
|
|
|
2.1×150mm
|
1.7μm
|
186003377
|
176001050
|
|
|
BEH 苯基
|
1.0×50mm
|
1.7μm
|
186002882
|
176000905
|
|
1.0×100mm
|
1.7μm
|
186002883
|
176000906
|
|
|
1.0×150mm
|
1.7μm
|
186003375
|
176001047
|
|
|
2.1×30mm
|
1.7μm
|
186003911
|
176001311
|
|
|
2.1×50mm
|
1.7μm
|
186002884
|
176000907
|
|
|
2.1×100mm
|
1.7μm
|
186002885
|
176000908
|
|
|
2.1×150mm
|
1.7μm
|
186003378
|
176001051
|
|
|
BEH HILIC
|
1.0×50mm
|
1.7μm
|
186003457
|
176001089
|
|
1.0×100mm
|
1.7μm
|
186003458
|
176001090
|
|
|
1.0×150mm
|
1.7μm
|
186003459
|
176001091
|
|
|
2.1×50mm
|
1.7μm
|
186003460
|
176001092
|
|
|
2.1×100mm
|
1.7μm
|
186003461
|
176001093
|
|
|
2.1×150mm
|
1.7μm
|
186003462
|
176001094
|
|
|
HSS C18
|
1.0×50mm
|
1.8μm
|
186003529
|
176001121
|
|
1.0×100mm
|
1.8μm
|
186003530
|
176001122
|
|
|
1.0×150mm
|
1.8μm
|
186003531
|
176001123
|
|
|
2.1×30mm
|
1.8μm
|
186003987
|
176001398
|
|
|
2.1×50mm
|
1.8μm
|
186003532
|
176001124
|
|
|
2.1×100mm
|
1.8μm
|
186003533
|
176001125
|
|
|
2.1×150mm
|
1.8μm
|
186003534
|
176001126
|
|
|
HSS C18 SB
|
1.0×50mm
|
1.8μm
|
186004114
|
176001556
|
|
1.0×100mm
|
1.8μm
|
186004115
|
176001557
|
|
|
1.0×150mm
|
1.8μm
|
186004116
|
176001558
|
|
|
2.1×30mm
|
1.8μm
|
186004117
|
176001559
|
|
|
2.1×50mm
|
1.8μm
|
186004118
|
176001560
|
|
|
2.1×100mm
|
1.8μm
|
186004119
|
176001561
|
|
|
2.1×150mm
|
1.8μm
|
186004120
|
176001562
|
|
|
HSS T3
|
1.0×50mm
|
1.8μm
|
186003535
|
176001127
|
|
1.0×100mm
|
1.8μm
|
186003536
|
176001129
|
|
|
1.0×150mm
|
1.8μm
|
186003537
|
176001375
|
|
|
2.1×30mm
|
1.8μm
|
186003944
|
176001130
|
|
|
2.1×50mm
|
1.8μm
|
186003538
|
176001131
|
|
|
2.1×100mm
|
1.8μm
|
186003539
|
176001132
|
|
|
2.1×150mm
|
1.8μm
|
186003540
|
176001133
|
|
描述
|
規格
|
貨號
|
|
高至低 pH 、*寬的分離選擇性 UPLC 柱組合包
|
2.1 x 50 mm
|
176001042
|
|
BEH C18, BEH C8, BEH Shield RP18, BEH Phenyl
|
2.1 x 100 mm
|
176001043
|
|
UPLC 方法開發柱組合包
|
2.1 x 50 mm
|
176001603
|
|
BEH C18, BEH Shield RP18, BEH Phenyl, HSS T3
|
2.1 x 100 mm
|
176001604
|
|
USP L1 UPLC 柱組合包
|
2.1 x 50 mm
|
176001605
|
|
BEH C18, BEH Shield RP18, HSS C18, HSS T3
|
2.1 x 100 mm
|
176001606
|
|
質譜分析用 UPLC 柱組合包
|
2.1 x 50 mm
|
176001607
|
|
BEH C18, HSS C18, HSS C18 SB, HSS T3
|
2.1 x 100 mm
|
176001608
|
|
低 pH、*寬的分離選擇性 UPLC 柱組合包
|
2.1 x 50 mm
|
176001609
|
|
BEH Shield RP18, BEH Phenyl, HSS C18, HSS C18 SB
|
2.1 x 100 mm
|
176001610
|
|
鍵合相
|
規格
|
粒徑
|
貨號
|
|
BEH C18
|
2.1 x 50 mm
|
1.7 μm
|
186004044
|
|
2.1 x 100 mm
|
1.7 μm
|
186004045
|
|
|
BEH C8
|
2.1 x 50 mm
|
1.7 μm
|
186004046
|
|
2.1 x 100 mm
|
1.7 μm
|
186004047
|
|
|
BEH Shield RP18
|
2.1 x 50 mm
|
1.7 μm
|
186004048
|
|
2.1 x 100 mm
|
1.7 μm
|
186004049
|
|
|
BEH 苯基
|
2.1 x 50 mm
|
1.7 μm
|
186004050
|
|
2.1 x 100 mm
|
1.7 μm
|
186004052
|
|
|
BEH HILIC
|
2.1 x 50 mm
|
1.7 μm
|
186004053
|
|
2.1 x 100 mm
|
1.7 μm
|
186004054
|
|
|
HSS C18
|
2.1 x 50 mm
|
1.8 μm
|
186004057
|
|
2.1 x 100 mm
|
1.8 μm
|
186004058
|
|
|
HSS C18 SB
|
2.1 x 50 mm
|
1.8 μm
|
186004137
|
|
2.1 x 100 mm
|
1.8 μm
|
186004138
|
|
|
HSS T3
|
2.1 x 50 mm
|
1.8 μm
|
186004055
|
|
2.1 x 100 mm
|
1.8 μm
|
186004056
|
|
鍵合相
|
規格
|
粒徑
|
部件號
|
|
BEH C18
|
2.1 x 5 mm
|
1.7 μm
|
186003975
|
|
BEH Shield RP18
|
2.1 x 5 mm
|
1.7 μm
|
186003977
|
|
BEH C8
|
2.1 x 5 mm
|
1.7 μm
|
186003978
|
|
BEH 苯基
|
2.1 x 5 mm
|
1.7 μm
|
186003979
|
|
BEH HILIC
|
2.1 x 5 mm
|
1.7 μm
|
186003980
|
|
HSS C18
|
2.1 x 5 mm
|
1.8 μm
|
186003981
|
|
HSS C18 SB
|
2.1 x 5 mm
|
1.8 μm
|
186004136
|
|
HSS T3
|
2.1 x 5 mm
|
1.8 μm
|
186003976
|
